Regulatory
pfic_mut

Part:BBa_K3790012:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-01)


The simplest form of a mutated fic promoter, a medium σS promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part. 

F_fic2_1035	5-AACCCGGATTTGCCGTCTATACTTGTGGCACTAGAGAAAGAGGAGAAATACTAGATGC
R_fic2_1907	5-ATAGACGGCAAATCCGGGTTACGCCGGGCTCCCACTCCACCTGAACC


Source

References